@prefix schema: .
@prefix gndo: .
@prefix lib: .
@prefix owl: .
@prefix xsd: .
@prefix skos: .
@prefix rdfs: .
@prefix editeur: .
@prefix geo: .
@prefix umbel: .
@prefix naf: .
@prefix sf: .
@prefix rdau: .
@prefix bflc: .
@prefix thesoz: .
@prefix dcterms: .
@prefix isbd: .
@prefix foaf: .
@prefix mesh: .
@prefix ram: .
@prefix mo: .
@prefix marcRole: .
@prefix agrelon: .
@prefix dcmitype: .
@prefix nsogg: .
@prefix dnbt: .
@prefix dbp: .
@prefix embne: .
@prefix rdf: .
@prefix dnb_intern: .
@prefix madsrdf: .
@prefix v: .
@prefix cidoc: .
@prefix dcatde: .
@prefix ebu: .
@prefix wdrs: .
@prefix bibo: .
@prefix gbv: .
@prefix agrovoc: .
@prefix lcsh: .
@prefix dc: .
a bibo:Issue;
dcterms:medium ;
rdau:P60049 ;
rdau:P60050 ;
rdau:P60048 ;
dc:identifier "(DE-101)1328634825";
umbel:isLike , ;
dc:identifier "(OCoLC)1189325787";
dcterms:language ;
a bibo:Article;
dc:title "Apparent isocitrate lyase activity in Leishmania amazonensis";
dcterms:creator _:node1k0rmm5k0x124896444 .
_:node1k0rmm5k0x124896444 gndo:preferredName "Hernández-Chinea, Concepción" .
marcRole:aut _:node1k0rmm5k0x124896445 .
_:node1k0rmm5k0x124896445 gndo:preferredName "Hernández-Chinea, Concepción" .
marcRole:aut _:node1k0rmm5k0x124896446 .
_:node1k0rmm5k0x124896446 gndo:preferredName "Maimone, Laura" .
marcRole:aut _:node1k0rmm5k0x124896447 .
_:node1k0rmm5k0x124896447 gndo:preferredName "Campos, Yelitza" .
marcRole:aut _:node1k0rmm5k0x124896448 .
_:node1k0rmm5k0x124896448 gndo:preferredName "Mosca, Walter" .
marcRole:aut _:node1k0rmm5k0x124896449 .
_:node1k0rmm5k0x124896449 gndo:preferredName "Romero, Pedro J." .
bibo:pageStart "701";
bibo:pageEnd "707";
dcterms:extent "7 S.";
dcterms:isPartOf ;
dcterms:description "Abstract: Early reports have demonstrated the occurrence of glyoxylate cycle enzymes in several Leishmania species. However, these results have been underestimated because genes for the two key enzymes of the cycle, isocitrate lyase (ICL) and malate synthase (MS), are not annotated in Leishmania genomes. We have re-examined this issue in promastigotes of Leishmania amazonensis. Enzyme activities were assayed spectrophotometrically in cellular extracts and characterized partially. A 40 kDa band displaying ICL activity was visualized on zymograms of the extracts. By immunoblotting with mouse antibodies against ICL from Bacillus stearothermophilus, a band of approximately 40 kDa was identified, coincident with the relative molecular mass of the activity band revealed on zymograms. Indirect immunofluorescence of intact promastigotes showed that the recognized antigen is distributed as a punctuated pattern, mainly distributed beneath the subpellicular microtubules, over a diffused cytoplasmic stain. These results clearly demonstrate the existence of an apparent ICL activity in L. amazonensis promastigotes, which is associated to a 40 kDa polypeptide and distributed both diffused and as punctuate aggregates in the cytoplasm. The relevance of this activity is discussed."@de;
wdrs:describedby .
dcterms:license ;
dcterms:modified "2024-05-07T21:44:42.000"^^xsd:dateTime .
dcterms:issued "2017";
bibo:issue "62.2017, 4";
owl:sameAs .