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a bibo:Issue;
dcterms:medium ;
rdau:P60049 ;
rdau:P60050 ;
rdau:P60048 ;
dc:identifier "(DE-101)1328726681";
umbel:isLike , ;
dcterms:language ;
a bibo:Article;
dc:title "Oligopeptidase B-2 from Leishmania amazonensis with an unusual C-terminal extension";
dcterms:creator _:node1jrarau8gx42327933 .
_:node1jrarau8gx42327933 gndo:preferredName "Matos Guedes, Herbert" .
marcRole:aut _:node1jrarau8gx42327934 .
_:node1jrarau8gx42327934 gndo:preferredName "Matos Guedes, Herbert" .
marcRole:aut _:node1jrarau8gx42327935 .
_:node1jrarau8gx42327935 gndo:preferredName "Carvalho, Rafael" .
marcRole:aut _:node1jrarau8gx42327936 .
_:node1jrarau8gx42327936 gndo:preferredName "Oliveira Gomes, Daniel" .
marcRole:aut _:node1jrarau8gx42327937 .
_:node1jrarau8gx42327937 gndo:preferredName "Rossi-Bergmann, Bartira" .
marcRole:aut _:node1jrarau8gx42327938 .
_:node1jrarau8gx42327938 gndo:preferredName "De-Simone, Salvatore" .
bibo:pageStart "197";
bibo:pageEnd "204";
dcterms:extent "8 S.";
dcterms:isPartOf ;
dcterms:description "Abstract: The oligopeptidase B serine protease is an important virulence factor and therapeutic target in Trypanosoma infections. Recently, the Leishmania major Genome Project identified a new oligopeptidase B that was denominated oligopeptidase B-like, herein named oligopeptidase B-2. In this study, a complete open reading frame of oligopeptidase B-2 from Leishmania amazonensis (PH8 strain) was amplified by PCR using primers designed for the oligopeptidase B-2 gene of L. major. The 2,715 bp fragment coded for a protein of 905 amino acids with a predicted molecular mass of 103,918.9 Da and theoretical pI of 5.82. The encoded protein displayed ∼96% identity with L. major and ∼75% identity with Trypanosoma cruzi and T. brucei oligopeptidases B-2, and ∼21% identity with Escherichia coli and L. amazonensis classical oligopeptidase B. An unusual C-terminal extension was found in relation to the classical trypanosomatid oligopeptidase B. By sequence alignment, we determined a catalytic triad (Ser 629, Asp 717 and His 758), S1 subsite (Glu 674 and Glu 676) and suggest a difference in the S2 subsite of L. amazonensis oligopeptidase B-2. We also found that the oligopeptidase B-2 gene is expressed in all cycle stages of L. amazonensis. A phylogenetic analysis indicated that oligopeptidase B-2 is a new member of oligopeptidase B."@de;
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dcterms:modified "2024-05-08T21:37:29.000"^^xsd:dateTime .
dcterms:issued "2008";
bibo:issue "53.2008, 2";
owl:sameAs .